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Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR activation may...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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Related Experiment Video

Updated: May 23, 2026

Stimulation of Notch Signaling in Mouse Osteoclast Precursors
08:01

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

miRNA-34c regulates Notch signaling during bone development.

Yangjin Bae1, Tao Yang, Huan-Chang Zeng

  • 1Department of Molecular and Human Genetics, Baylor College of Medicine,One Baylor Plaza, Houston, TX 77030, USA.

Human Molecular Genetics
|April 14, 2012
PubMed
Summary

MicroRNA 34c (miR-34c) is crucial for bone homeostasis, regulating osteoblast and osteoclast activity. Its dysregulation contributes to osteoporosis and may play a role in osteosarcoma development.

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Last Updated: May 23, 2026

Stimulation of Notch Signaling in Mouse Osteoclast Precursors
08:01

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis
07:26

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

Published on: April 1, 2022

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Bone Biology

Background:

  • Bone homeostasis relies on balanced osteoblast and osteoclast activity.
  • MicroRNAs (miRNAs) are key regulators of cellular differentiation and signaling pathways.
  • The role of specific miRNAs in osteoblastogenesis and bone disease is an active area of research.

Purpose of the Study:

  • To investigate the role of microRNA 34 (miR-34) in osteoblast differentiation and bone homeostasis.
  • To elucidate the molecular targets and mechanisms of miR-34 in bone cells.
  • To explore the potential involvement of miR-34 in bone pathologies like osteoporosis and osteosarcoma.

Main Methods:

  • BMP2 induction of miR-34 during osteoblast differentiation.
  • In vivo studies using mice with osteoblast-specific gain of miR-34c.
  • Analysis of osteoblast proliferation, mineralization, and osteoclastogenesis.
  • Target validation of Notch signaling components (Notch1, Notch2, Jag1) in osteoblasts.

Main Results:

  • miR-34 was significantly induced by BMP2 during osteoblast differentiation.
  • Osteoblast-specific gain of miR-34c in mice led to age-dependent osteoporosis.
  • Defective osteoblast mineralization and proliferation, coupled with increased osteoclastogenesis, were observed.
  • miR-34c directly targeted and regulated components of the Notch signaling pathway in osteoblasts.
  • miR-34c influenced osteoclast differentiation in a non-cell-autonomous manner.

Conclusions:

  • miR-34c is critical for osteoblastogenesis by regulating Notch signaling, thereby maintaining bone homeostasis.
  • miR-34c's post-transcriptional regulation of Notch signaling in osteoblasts impacts osteoblast progenitor proliferation.
  • Understanding the miR-34 and Notch signaling interplay is vital for developing therapies for bone diseases and osteosarcoma.