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Correlation between serum matrix metalloproteinase and antigenemia levels in patients infected with rotavirus
Yoshiki Kawamura1, Ken Sugata, Hidetaka Nakai
1Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
Abstract:
Rotavirus (RV) antigenemia has been reported in patients with gastroenteritis; however, the exact mechanism remains unclear. In order to elucidate the mechanism of RV antigenemia, an association between RV antigenemia and matrix metalloproteinase (MMP) were analyzed. The object of this study was to elucidate the role of MMPs and tissue inhibitors of metalloproteinases (TIMPs) in the pathogenesis of RV antigenemia. Forty children admitted to hospital with RV gastroenteritis were enrolled in this study. Paired serum samples were collected at the time of admission and discharge. Enzyme-linked immunosorbent assays (ELISA) were used to detect serum concentrations of viral antigens, MMP-1, -2, -9, -13, TIMP -1, and -2. Cytokines were measured using flow cytometric beads array. RV antigens were significantly higher in serum collected at the time of admission than discharge (P < 0.001). MMP-9 concentrations were significantly higher in serum collected at the time of admission than discharge (P < 0.001). MMP-2 concentrations were significantly lower in serum collected at the time of admission than discharge (P < 0.001). A weak but a significantly positive association (P = 0.034) was observed between RV antigen and MMP-9 in serum collected at the time of admission, and inverse association was observed between RV antigen and MMP-2. In addition, a weak but significantly positive association (P = 0.002) was observed between IL-6 and MMP-9. These data suggest that MMPs may contribute to the pathogenesis of RV antigenemia.
Insights
Matrix metalloproteinases (MMPs) may play a role in rotavirus (RV) antigenemia during gastroenteritis. This study found higher MMP-9 levels and a positive association with RV antigen in children with RV gastroenteritis.
Area of Science:
- Virology
- Immunology
- Biochemistry
Background:
- Rotavirus (RV) antigenemia is observed in gastroenteritis, but its mechanism is not fully understood.
- Matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) are involved in tissue remodeling and inflammation.
Purpose of the Study:
- To investigate the association between RV antigenemia and MMPs/TIMPs in children with RV gastroenteritis.
- To elucidate the role of MMPs and TIMPs in the pathogenesis of RV antigenemia.
Main Methods:
- Enrolled 40 children with RV gastroenteritis, collecting paired serum samples at admission and discharge.
- Used ELISA to quantify viral antigens, MMPs (1, 2, 9, 13), and TIMPs (1, 2).
- Measured cytokines using flow cytometric bead array.
Main Results:
- Significantly higher RV antigen and MMP-9 levels at admission compared to discharge (P < 0.001).
- Significantly lower MMP-2 levels at admission compared to discharge (P < 0.001).
- Positive association between RV antigen and MMP-9 (P = 0.034), and IL-6 and MMP-9 (P = 0.002).
Conclusions:
- MMPs, particularly MMP-9, may contribute to the pathogenesis of RV antigenemia.
- The findings suggest a link between MMP activity and RV antigen presence during gastroenteritis.
