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Membrane microdomain may be a platform for immune signaling

Yiping Qi1, Fumiaki Katagiri

  • 1Department of Plant Biology, Microbial and Plant Genomics Institute, University of Minnesota, St. Paul, MN, USA.

Plant Signaling & Behavior
|April 14, 2012
PubMed

Insights

Arabidopsis hypersensitive induced reaction (AtHIR) proteins are involved in plant immunity. This study shows AtHIR proteins associate with both R proteins and pattern recognition receptors (PRRs), suggesting they facilitate plant immune signaling.

Area of Science:

  • Plant immunity
  • Molecular plant-pathogen interactions
  • Cellular signaling

Background:

  • Arabidopsis RPS2 is a nucleotide-binding leucine-rich repeats (NB-LRR) type disease resistance protein.
  • Arabidopsis hypersensitive induced reaction (AtHIR) proteins are associated with RPS2 and enriched in membrane microdomains, suggesting a role in immune signaling.

Purpose of the Study:

  • To investigate the role of AtHIR proteins in plant immune signaling.
  • To determine if pattern recognition receptors (PRRs) also associate with AtHIR proteins.

Main Methods:

  • Transient expression in Nicotiana benthamiana
  • Biochemical analyses
  • Genetic analyses

Main Results:

  • AtHIR proteins are physically associated with the pattern recognition receptor (PRR) FLS2.
  • This association occurs in a transient expression system.

Conclusions:

  • Plasma membrane (PM) microdomains may serve as a platform for both R proteins and PRRs.
  • AtHIR proteins might facilitate the activation of both types of immune receptors in plant defense signaling.

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