Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 23, 2026

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
09:24

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

Development of multilayer constructs for tissue engineering.

N M S Bettahalli1, N Groen, H Steg

  • 1Membrane Technology Group, Faculty of Science and Technology, PO Box 217, 7500 AE, Enschede, The Netherlands.

Journal of Tissue Engineering and Regenerative Medicine
|April 14, 2012
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First Measurement of Time-Dependent CP Violation in the Flavor-Changing Neutral-Current Decay B^{0}→K_{S}^{0}μ^{+}μ^{-}.

Physical review letters·2026
Same author

Measurement of the Top-Quark Production Cross Section and Charge Asymmetry at LHCb.

Physical review letters·2026
Same author

Searches for B^{0}→K^{+}π^{-}τ^{+}τ^{-} and B_{s}^{0}→K^{+}K^{-}τ^{+}τ^{-} Decays.

Physical review letters·2026
Same author

First Evidence of the B_{s}^{0}→K^{-}π^{+}γ Decay.

Physical review letters·2026
Same author

Precision Measurement of CP Violation and Branching Fractions in B^{±}→K_{S}^{0}h^{±} (h=π, K) Decays and Search for the Rare Decay B_{c}^{±}→K_{S}^{0}K^{±}.

Physical review letters·2026
Same author

An In Vitro System for Studying Toxin Transport Across Hemodialysis Membranes.

Artificial organs·2026

This study developed a novel multilayer tissue construct using electrospun sheets and hollow fiber membranes. This innovative scaffold enhances nutrient and oxygen delivery, promoting cell growth for complex tissue engineering.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Tissue engineering aims to create living substitutes for damaged tissues or organs, offering permanent cures.
  • Current limitations include challenges in creating clinically sized tissues with complex architectures due to nutrient/oxygen delivery issues and scaffold limitations.

Purpose of the Study:

  • To develop and test a novel multilayer tissue construct for improved tissue engineering.
  • To address limitations in nutrient and oxygen supply for larger, complex tissue development.

Main Methods:

  • Fabrication of a multilayer construct by rolling poly(l-lactic acid) (PLLA) sheets seeded with C2C12 pre-myoblast cells around a porous multibore hollow fiber (HF) membrane.
  • Utilizing the HF membrane as an additional nutrient/oxygen source with controllable low shear stress.
Keywords:
bioreactorelectro spinninghollow fibremultilayer scaffoldtissue engineering

More Related Videos

A Facile and Eco-friendly Route to Fabricate Poly(Lactic Acid) Scaffolds with Graded Pore Size
13:46

A Facile and Eco-friendly Route to Fabricate Poly(Lactic Acid) Scaffolds with Graded Pore Size

Published on: October 17, 2016

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures
05:52

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures

Published on: September 27, 2019

Related Experiment Videos

Last Updated: May 23, 2026

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
09:24

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

A Facile and Eco-friendly Route to Fabricate Poly(Lactic Acid) Scaffolds with Graded Pore Size
13:46

A Facile and Eco-friendly Route to Fabricate Poly(Lactic Acid) Scaffolds with Graded Pore Size

Published on: October 17, 2016

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures
05:52

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures

Published on: September 27, 2019

  • Applying dynamic perfusion through the HF lumen and around the construct in a bioreactor.

    • Main Results:

    • Demonstrated successful cell proliferation and homogenous cell distribution across multiple layers.
    • Observed cell migration within the multilayer construct, indicating construct integration potential.
    • The developed system effectively delivered nutrients and oxygen to cells within the construct.

    Conclusions:

    • The developed multilayer tissue construct, utilizing a porous hollow fiber membrane, shows significant potential for engineering clinically relevant, complex tissues.
    • Dynamic perfusion and integrated nutrient delivery are key factors for successful cell proliferation and distribution in engineered tissues.
    • This approach offers a promising strategy for overcoming current challenges in developing thick and architecturally complex tissue substitutes.