Related Experiment Video
Updated: May 23, 2026
Analysis of Cardiac Contractile Dysfunction and Ca2+ Transients in Rodent Myocytes
Published on: May 25, 2022
Morphology and contractility in cardiomyocytes of rats with low exercise performance
Judson Fonseca Quintão-Júnior1, Antônio José Natali, Miguel Araújo Carneiro-Júnior
1Universidade Federal de Viçosa, Viçosa, MG, Brazil.
Background:
Aerobic capacity is essential to physical performance, and low aerobic capacity is related to the triggering of various cardiovascular diseases.
Objective:
To compare the morphology and contractility of isolated rat cardiomyocytes with low performance and standard performance for exercise.
Methods:
Wistar rats with 10 weeks of age underwent a protocol of treadmill running to fatigue, and were divided into two groups: Low Performance (LP) and Standard Performance (SP). Then, the animals were sacrificed, the heart was quickly removed and, by means of enzymatic dissociation, left ventricular cardiomyocytes were isolated. The cell and sarcomeres length and width of cardiomyocytes were measured using an edge detection system. The isolated cardiomyocytes were electrically stimulated at 1 and 3 Hz and cell contraction was measured by registering the change of their length.
Results:
The cell length was shorter in the LP group (157.2 ± 1.3 µm; p < 0.05) compared to SP (161.4 ± 1.3 µm), and the same result was observed for the volume of cardiomyocytes (LP, 25.5 ± 0.4 vs. SP, 26.8 ± 0.4 pL; p < 0.05). The time to peak contraction (LP, 116 ± 1 vs. SP 111 ± 2 ms) and total relaxation (LP, 143 ± 3 vs. SP 232 ± 3 ms) were higher in the LP group.
Conclusion:
We conclude that left ventricular myocytes of animals with low performance for exercise are smaller than animals with standard performance. In addition to that, they present losses in contractile capacity.
