Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Ligand Binding and Linkage00:49

Ligand Binding and Linkage

Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence the...
Conserved Binding Sites01:49

Conserved Binding Sites

Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally analyses the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Reliable Prediction Method for the Pedicle Subtraction Osteotomy Angle in Ankylosing Spondylitis-Related Thoracolumbar Kyphosis: A Comparative Analysis with Existing Approaches.

Clinics in orthopedic surgery·2026
Same author

Computational models for the classification of antibody specificity using heavy chain features.

PloS one·2026
Same author

Determination of Cell Viability-Acridine Orange/Propidium Iodide (AO/PI) Staining Method.

Cell proliferation·2026
Same author

Tunneling Nanotube-Mediated Filamin A Transport Mechanosensitively Programs Osteoclastogenesis in Myeloma Bone Disease.

Blood·2026
Same author

Surgical algorithms for ankylosing spondylitis-related pseudarthrosis : adapting to radiological and clinical variations.

Bone & joint open·2026
Same author

Mosaic phenotypic evolution underlies the adaptive success of water-surface colonization in Gerromorpha.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

[Epigenetic regulator DOT1L controls neuronal amyloid pre-cursor protein expression via the p38 mediated mitochondrial fission-fusion homeostasis axis].

Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences·2026
Same journal

[Association between blood mitochondrial DNA copy number and adverse pregnancy outcomes: a Mendelian randomization analysis].

Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences·2026
Same journal

[Network pharmacology-based investigation of the therapeutic efficacy and molecular targets of Xiangsha Liujunzi Decoction in thyroid cancer].

Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences·2026
Same journal

[En-bloc resection of thyroid cancer using recurrent laryngeal nerve tunnel dissection combined with mesangectomy: a retro-spective analysis of 174 cases].

Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences·2026
Same journal

[Methyltransferase-like protein 7B promotes glycolysis and malignant progression in papillary thyroid carcinoma via the USP28/HIF-1α axis].

Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences·2026
Same journal

[Preparation of all-trans retinoic acid and irinotecan co-loaded liposomes and their <i>in vitro</i> evaluation against anaplastic thyroid cancer].

Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences·2026
See all related articles

Related Experiment Video

Updated: May 23, 2026

Understanding the Development of Compensatory Pathways in a Mutant Malaria Parasite Harbouring Hypomorphic Allele of Plant-Like Kinases
09:13

Understanding the Development of Compensatory Pathways in a Mutant Malaria Parasite Harbouring Hypomorphic Allele of Plant-Like Kinases

Published on: November 22, 2024

[K83 site affects PICK1 PDZ binding ability].

Yong Feng1, Mu Qiao, Yu-ting Lu

  • 1Institute of neuroscience, Zhejiang University School of Medicine, Hangzhou 310058, China.

Zhejiang Da Xue Xue Bao. Yi Xue Ban = Journal of Zhejiang University. Medical Sciences
|April 14, 2012
PubMed
Summary
This summary is machine-generated.

The K83 site on PICK1 is crucial for binding with GluR2. Mutations at this site alter the interaction between PICK1 and GluR2, affecting their cellular distribution.

More Related Videos

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Related Experiment Videos

Last Updated: May 23, 2026

Understanding the Development of Compensatory Pathways in a Mutant Malaria Parasite Harbouring Hypomorphic Allele of Plant-Like Kinases
09:13

Understanding the Development of Compensatory Pathways in a Mutant Malaria Parasite Harbouring Hypomorphic Allele of Plant-Like Kinases

Published on: November 22, 2024

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Area of Science:

  • Molecular biology
  • Protein-protein interactions
  • Neuroscience

Context:

  • PICK1 (Protein Interacting with C Kinase 1) is a scaffolding protein.
  • The PDZ domain of PICK1 interacts with the C-terminal of various proteins, including GluR2.
  • This interaction is critical for synaptic plasticity and receptor trafficking.

Purpose:

  • To investigate the specific role of the K83 site within the PICK1 PDZ domain in its interaction with the GluR2 C-terminus.
  • To determine how altering the K83 site affects the binding affinity and cellular localization of PICK1 and GluR2.

Summary:

  • Computational modeling and site-directed mutagenesis were used to study the K83 site in PICK1's PDZ domain.
  • Mutations at K83 significantly altered the distribution of PICK1 and GluR2 in HEK293T cells.
  • The K83 site is essential for the PICK1-GluR2 interaction, influencing binding energy and molecular interactions.

Impact:

  • This research elucidates a key molecular determinant for PICK1-GluR2 complex formation.
  • Understanding this interaction is vital for comprehending AMPA receptor regulation and synaptic function.
  • Findings could inform therapeutic strategies targeting neurological disorders involving PICK1 and GluR2.