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[Influence of isoliensinine on activity of CYP3A in rats]
1College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
Objective:
To investigate the influence of isoliensinine (IL) on CYP3A enzyme activity.
Methods:
A mixture metabolic system of liver microsome enzymes in vitro was developed. A HPLC method to test the metabolic activity of CYP3A was established with testosterone as a probe. The activities of CYP3A enzymes were measured with different IL concentrations and incubation time with testosterone.
Results:
In mixture metabolic system of liver microsome enzymes, the best incubation concentration of testosterone was 200 μmol/L, the best incubation time was 210 min, in this condition the IC₅₀ of IL for CYP3A inhibition was >1 000 μmol/L.
Conclusion:
No significant interaction between IL and CYP3A is detected, which indicates that IL might be used with CYP 3A enzyme substrates.
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