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Related Experiment Video

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Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
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ALDH marks leukemia stem cell.

Angela G Fleischman1

  • 1Oregon Health & Science University, USA.

Blood
|April 14, 2012
PubMed
Summary
This summary is machine-generated.

Researchers identified a specific leukemia stem cell (LSC) population in acute myeloid leukemia (AML) bone marrow using aldehyde dehydrogenase (ALDH) activity. This ALDH-intermediate cell group predicts minimal residual disease after treatment.

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Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia

Published on: September 5, 2019

Area of Science:

  • Hematology
  • Cancer Biology
  • Stem Cell Research

Background:

  • Acute myeloid leukemia (AML) is characterized by a hierarchical cell organization.
  • Leukemia stem cells (LSCs) are crucial for disease initiation and relapse.
  • The CD34(+)CD38(-) compartment is known to contain stem and progenitor cells, including LSCs.

Purpose of the Study:

  • To investigate the role of aldehyde dehydrogenase (ALDH) activity in characterizing LSCs within the CD34(+)CD38(-) compartment in AML.
  • To determine if ALDH activity can identify a specific LSC population.
  • To assess the prognostic significance of this identified population regarding minimal residual disease (MRD).

Main Methods:

  • Analysis of bone marrow samples from AML patients.
  • Utilizing flow cytometry to assess cell surface markers (CD34, CD38) and intracellular aldehyde dehydrogenase (ALDH) activity.
  • Correlating the presence and persistence of specific cell populations with clinical outcomes and MRD status.

Main Results:

  • A distinct subpopulation with intermediate aldehyde dehydrogenase (ALDH(int)) activity was identified within the CD34(+)CD38(-) compartment.
  • This ALDH(int) population was found to contain leukemia stem cells (LSCs).
  • The persistence of ALDH(int) cells post-therapy was a significant indicator of clinically relevant minimal residual disease (MRD).

Conclusions:

  • Aldehyde dehydrogenase activity provides a valuable tool for subdividing the CD34(+)CD38(-) compartment in AML.
  • The ALDH(int) population represents a key LSC reservoir in AML.
  • Monitoring ALDH(int) cell persistence is a promising strategy for assessing MRD and predicting relapse in AML patients.