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Updated: May 23, 2026
HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis
Published on: November 11, 2022
[Study on fingerprint of semen cassiae, ultramicro powder and powder particle]
Yu-Lian Tang1, Yi-Zeng Liang, Hua-Xiu Zhang
1Chemistry and Chemical Engineering Department of Huaihua University, Huaihua 418008, China. tangyulian0824@163.com
Objective:
To establish HPLC fingerprint of Semen Cassiae.
Methods:
Analysis on a Phenomenex C18 (4.6 mm x 250 mm, 5 microm) column eluted with phases containing acetonitrile and 0.1% phosphoric acid in water in gradient mode. The flow rate was 0.8 mL/min and the detection wavelength was 280 nm. The temperature of column was 30 degrees C. Data of 12 batches of Semen Cassiae, ultramicro powder and powder particle from different sources were analysed by "similarity evaluation for chromatographic Fingerprint of Traditional Chinese Medicine" software, and the similarity of them was compared.
Results:
Twenty-seven common peaks were identified in the chromatograms,and all the common peaks were separated effectively. Fingerprint of ultramicro powder and Semen Cassiae was basic similar, but fingerprint of powder particle and Semen Cassiae was obviously different.
Conclusion:
The method is precise, repeatabe and stable. It can be used to identity and evaluate the quality of Semen Cassiae, and provide important theoretical foundation for clinical applications of Semen Cassiae.
