Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An Integrated Skin Cell Atlas Decodes the Pilosebaceous Unit.

The Journal of investigative dermatology·2026
Same author

Psychiatric and behavioral symptoms after pediatric herpes simplex virus type 1 encephalitis: An exploratory case series.

Bulletin of the Menninger Clinic·2026
Same author

The Liver S9 Proteome of Rat and Hamster: Global Profiling and Targeted Cytochrome P450 Quantification Reveal Induction-Responsive Remodeling.

Journal of proteome research·2026
Same author

Worked examples, cognitive aptitudes and the self-explanation mechanism - A replication.

The British journal of educational psychology·2026
Same author

Unraveling the complexity of skin's biological aging utilizing epigenetic clocks.

Clinical epigenetics·2026
Same author

Sunscreen application substantially mitigates molecular perturbations induced by repetitive UV exposure and maintains healthy skin.

Scientific reports·2026

Related Experiment Video

Updated: May 23, 2026

A Workflow to Quantitatively Determine Age-Related Macular Degeneration Lesion-Specific Variations in Fundus Autofluorescence
08:54

A Workflow to Quantitatively Determine Age-Related Macular Degeneration Lesion-Specific Variations in Fundus Autofluorescence

Published on: May 26, 2023

Approach to quantify human dermal skin aging using multiphoton laser scanning microscopy.

Stefan Puschmann1, Christian-Dennis Rahn, Horst Wenck

  • 1Beiersdorf AG, Research & Development, Unnastrasse 48, 20245 Hamburg, Germany.

Journal of Biomedical Optics
|April 17, 2012
PubMed
Summary

A new elastin-to-collagen ratio (ELCOR) parameter quantifies skin aging noninvasively. This method reveals significant age-related dermal changes and highlights sun damage effects on skin extracellular matrix.

More Related Videos

Enhancement of Facial Rejuvenation Through a Combination of 1565 nm Non-Ablative Fractional Laser with 30% Supramolecular Salicylic Acid
03:47

Enhancement of Facial Rejuvenation Through a Combination of 1565 nm Non-Ablative Fractional Laser with 30% Supramolecular Salicylic Acid

Published on: September 27, 2024

Visualizing Scar Development Using SCAD Assay - An Ex-situ Skin Scarring Assay
07:40

Visualizing Scar Development Using SCAD Assay - An Ex-situ Skin Scarring Assay

Published on: April 28, 2022

Related Experiment Videos

Last Updated: May 23, 2026

A Workflow to Quantitatively Determine Age-Related Macular Degeneration Lesion-Specific Variations in Fundus Autofluorescence
08:54

A Workflow to Quantitatively Determine Age-Related Macular Degeneration Lesion-Specific Variations in Fundus Autofluorescence

Published on: May 26, 2023

Enhancement of Facial Rejuvenation Through a Combination of 1565 nm Non-Ablative Fractional Laser with 30% Supramolecular Salicylic Acid
03:47

Enhancement of Facial Rejuvenation Through a Combination of 1565 nm Non-Ablative Fractional Laser with 30% Supramolecular Salicylic Acid

Published on: September 27, 2024

Visualizing Scar Development Using SCAD Assay - An Ex-situ Skin Scarring Assay
07:40

Visualizing Scar Development Using SCAD Assay - An Ex-situ Skin Scarring Assay

Published on: April 28, 2022

Area of Science:

  • Dermatology
  • Biomedical Optics
  • Aging Research

Background:

  • Intrinsic and extrinsic aging impair extracellular skin structures.
  • Current assessment methods include subjective clinical evaluation and invasive histological/molecular analyses.
  • A noninvasive quantitative method is needed to assess dermal alterations.

Purpose of the Study:

  • To develop a novel parameter for noninvasive, quantitative determination of dermal skin alterations.
  • To utilize high-resolution three-dimensional multiphoton laser scanning microscopy (MPLSM) for this purpose.
  • To quantify structural differences in skin extracellular matrix between sun-exposed and sun-protected areas and across age groups.

Main Methods:

  • Employed MPLSM to record collagen-specific second harmonic generation and elastin-specific autofluorescence signals in human skin.
  • Recruited young and elderly volunteers with varying sun exposure histories.
  • Calculated the elastin-to-collagen ratio (ELCOR) parameter after image processing.

Main Results:

  • The ELCOR parameter significantly increases with age in volar forearm skin.
  • Elderly volunteers showed significantly higher ELCOR values in sun-exposed temple areas compared to sun-protected upper arm areas.
  • Demonstrated age-associated alterations in the skin's extracellular matrix.

Conclusions:

  • Introduced the ELCOR parameter as a novel, accurate, and noninvasive method for quantifying age-associated extracellular matrix changes in skin.
  • MPLSM technology enables precise measurement of dermal structural alterations.
  • ELCOR can differentiate between intrinsic aging and extrinsic (sun-induced) aging effects on skin structure.