Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Electrophoresis: Overview

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

Capillary Electrophoresis: Instrumentation

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Sample Injection Techniques.

Methods in molecular biology (Clifton, N.J.)·2018

Same author

AOTF-based multicolor fluorescence detection for short tandem repeat (STR) analysis in an electrophoretic microdevice.

Lab on a chip·2008

Same author

On-chip pumping for pressure mobilization of the focused zones following microchip isoelectric focusing.

Lab on a chip·2006

Same author

A fully integrated microfluidic genetic analysis system with sample-in-answer-out capability.

Proceedings of the National Academy of Sciences of the United States of America·2006

Same author

Multicolor fluorescence detection on an electrophoretic microdevice using an acoustooptic tunable filter.

Analytical chemistry·2006

Same author

On-chip pressure injection for integration of infrared-mediated DNA amplification with electrophoretic separation.

Lab on a chip·2006

Search research articles

Related Experiment Video

Updated: May 23, 2026

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

Sample introduction techniques for microchip electrophoresis: a review.

James M Karlinsey¹

¹Department of Chemistry, Penn State Berks, Reading, PA 19610-7009, United States. jmk48@psu.edu

Analytica Chimica Acta

|April 17, 2012

Summary

This summary is machine-generated.

Reliable sample introduction is crucial for microchip electrophoresis systems. This review covers pressure-driven and electrokinetic methods for sample injection in micro total analysis systems (μTAS).

More Related Videos

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Characterizing Exon Skipping Efficiency in DMD Patient Samples in Clinical Trials of Antisense Oligonucleotides

Characterizing Exon Skipping Efficiency in DMD Patient Samples in Clinical Trials of Antisense Oligonucleotides

Published on: May 7, 2020

Related Experiment Videos

Last Updated: May 23, 2026

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Characterizing Exon Skipping Efficiency in DMD Patient Samples in Clinical Trials of Antisense Oligonucleotides

Characterizing Exon Skipping Efficiency in DMD Patient Samples in Clinical Trials of Antisense Oligonucleotides

Published on: May 7, 2020

Area of Science:

Analytical Chemistry
Microfluidics
Biotechnology

Background:

Microfluidic analysis systems are increasingly used across various scientific disciplines.
Sample introduction into separation channels is a critical, yet often unchanged, component of these systems.
Effective sample injection is paramount for the performance of electrophoretic micro total analysis systems (μTAS).

Purpose of the Study:

To review and highlight various sample introduction methods for microchip electrophoresis.
To focus on techniques relevant to micro total analysis systems (μTAS) applications.
To discuss both pressure-driven and electrokinetic sample introduction strategies.

Main Methods:

Literature review of published methods for sample introduction in microchip electrophoresis.
Categorization of techniques into pressure-driven and electrokinetic approaches.
Analysis of methods specifically employed in micro total analysis systems (μTAS).

Main Results:

Diverse methods exist for sample introduction, impacting overall system performance.
Pressure-driven techniques offer controlled injection volumes.
Electrokinetic techniques leverage electric fields for sample manipulation.

Conclusions:

The choice of sample introduction method significantly influences the reliability and reproducibility of microchip electrophoresis.
Advancements in sample introduction are key to optimizing micro total analysis systems (μTAS).
Further research into novel injection techniques can enhance the capabilities of microfluidic devices.