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Updated: May 23, 2026

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Antigen-Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Mycoplasma pneumoniae
Published on: February 24, 2023
Optimized serodiagnosis of Mycoplasma pneumoniae infections
Roger Dumke1, Andreas Strubel, Camille Cyncynatus
1Institute of Medical Microbiology and Hygiene, Dresden University of Technology, Fetscherstrasse 74, Dresden, Germany. roger.dumke@tu-dresden.de
Diagnostic Microbiology and Infectious Disease
|April 17, 2012
Abstract:
Serologic methods are well established for the diagnosis of Mycoplasma pneumoniae infections in humans, but they are less sensitive than polymerase chain reaction (PCR). To improve their sensitivity, a new panel of antigens was tested. Compared with PCR results, up to 92% of PCR-positive patients were confirmed by our immunoblotting approach having a specificity between 92.6% and 100%.
