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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: May 23, 2026

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition
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Published on: February 9, 2024

Protein detection based on small molecule-linked DNA.

Ya Cao1, Sha Zhu, Jiacui Yu

  • 1Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai, China.

Analytical Chemistry
|April 17, 2012
PubMed
Summary
This summary is machine-generated.

A new electrochemical method uses DNA probes and nicking endonuclease-assisted amplification (NEA) for sensitive protein detection. This approach enables accurate quantification of proteins like folate receptor (FR) in biological samples.

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Area of Science:

  • Electrochemistry
  • Biotechnology
  • Molecular Diagnostics

Background:

  • Protein detection is crucial for disease diagnosis and monitoring.
  • Existing electrochemical methods often face challenges with sensitivity and specificity.
  • Novel strategies are needed for efficient and reliable protein quantification.

Purpose of the Study:

  • To develop a novel electrochemical method for sensitive protein detection.
  • To utilize small molecule-linked DNA and nicking endonuclease-assisted amplification (NEA).
  • To demonstrate the method's applicability for protein quantification in biological samples.

Main Methods:

  • Small molecule-linked DNA probes were designed to bind target proteins.
  • Protein binding protected DNA from exonuclease digestion, enabling hybridization to an electrode.
  • Nicking endonuclease-assisted amplification (NEA) was triggered, leading to signal amplification.

Main Results:

  • The electrochemical signal increased proportionally to the amount of target protein.
  • Folate receptor (FR) was quantified in a linear range of 0.3-15 ng/mL with a detection limit of 0.19 ng/mL.
  • The method successfully detected FR in serum samples and could be adapted for other proteins like streptavidin.

Conclusions:

  • A novel, sensitive electrochemical protein detection method based on NEA was established.
  • The method offers a versatile platform for quantifying various proteins by modifying DNA probes.
  • This technique shows significant potential for applications in clinical diagnostics and biosensing.