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Related Concept Videos

Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...

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Related Experiment Video

Updated: May 30, 2026

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses
11:48

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses

Published on: April 4, 2019

SV40 recombinants carrying rabbit beta-globin gene coding sequences.

D H Hamer, K D Smith, S H Boyer

    Cell
    |July 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    SV40 recombinants were created to study gene expression. These viruses demonstrated that a specific SV40 segment, likely the splicing region, is essential for producing stable messenger RNA and new proteins.

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    Area of Science:

    • Molecular Biology
    • Virology
    • Genetic Engineering

    Background:

    • Simian virus 40 (SV40) is a valuable tool for studying eukaryotic gene regulation.
    • Understanding viral regulatory sequences is crucial for gene therapy and biotechnology.

    Purpose of the Study:

    • To investigate the role of SV40 regulatory sequences in gene expression.
    • To assess the utility of SV40 recombinants for producing hybrid transcripts and new gene products.

    Main Methods:

    • Construction and propagation of two SV40 recombinant viruses.
    • Replacement of portions of the SV40 late gene region with a rabbit beta-globin coding sequence.
    • Analysis of viral transcript stability and protein synthesis using radioimmunoassay.

    Main Results:

    • One SV40 recombinant produced a stable SV40-globin hybrid transcript, which was translated into detectable beta-globin in infected monkey cells.
    • A second recombinant, lacking the RNA splicing region, failed to produce stable globin transcripts or detectable beta-globin.
    • These findings pinpoint a 500 bp SV40 segment, likely the splicing region, as critical for stable mRNA accumulation.

    Conclusions:

    • SV40 splicing regions are essential for the accumulation of stable viral late mRNA.
    • Hybrid transducing viruses serve as effective tools for characterizing viral regulatory elements.
    • SV40 recombinants can be utilized to synthesize novel gene products in cultured cells.