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Related Concept Videos

Chromatography: Introduction01:10

Chromatography: Introduction

Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
Chromatographic Methods: Classification01:12

Chromatographic Methods: Classification

Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
Chromatographic techniques are typically named by...
Principles Of Column Chromatography01:13

Principles Of Column Chromatography

The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
Chromatographic Methods: Terminology01:18

Chromatographic Methods: Terminology

Chromatography is an analytical technique widely used in fields such as chemistry, biology, environmental science, and pharmaceuticals to separate the components of a mixture and identify substances between them. The process of chromatography is based on the interactions between two distinct phases: the stationary phase and the mobile phase. The stationary phase is fixed in place by a supporting material, while the mobile phase moves over it, carrying the solutes. As the mobile phase travels,...
Types Of Column Chromatography01:29

Types Of Column Chromatography

The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
Chromatographic Resolution01:15

Chromatographic Resolution

In chromatography, a solute moves through a chromatographic column and tends to spread, forming a Gaussian-shaped band. The longer the solute spends in the column, the broader the band becomes. The broadening can lead to overlaps within the column, affecting separation effectiveness.
The effectiveness of separation can be evaluated by determining the level of separation between two neighboring peaks in a chromatogram, which represents the individual components of a sample.
In chromatography,...

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Related Experiment Video

Updated: May 23, 2026

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
11:44

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

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Progress in cell membrane chromatography.

L C He1, S C Wang, G D Yang

  • 1School of Medicine, Xi'an Jiaotong University, Xi'an, China.

Drug Discoveries & Therapeutics
|April 17, 2012
PubMed
Summary
This summary is machine-generated.

Cell membrane chromatography (CMC) is a bioaffinity technique for drug-receptor studies. This review covers its stationary phase, analytical system, and applications in screening herbal compounds.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Pharmacology

Background:

  • Cell membrane chromatography (CMC) was developed in 1996.
  • It is a bioaffinity chromatography technique.
  • CMC is valuable for drug-receptor interaction studies and screening compounds from medicinal herbs.

Purpose of the Study:

  • To review the characteristics of the cell membrane stationary phase (CMSP).
  • To describe the CMC analytical system.
  • To summarize the applications of CMC.

Main Methods:

  • Review of existing literature on CMC.
  • Analysis of the properties of CMSP.
  • Examination of CMC system configurations and applications.

Main Results:

  • Detailed characteristics of CMSP are presented.
  • The CMC analytical system is described.
  • Various applications of CMC are highlighted.

Conclusions:

  • CMC is an established and important bioaffinity chromatography method.
  • The review provides a comprehensive overview of CMC's components and uses.
  • CMC remains a key technique in drug discovery and research.