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Published on: May 8, 2015
Structure of the tetradecanucleotide d(CCCCGGTACCGGGG)2 as an A-DNA duplex
Pradeep Kumar Mandal1, Sarkarai Venkadesh, Namasivayam Gautham
1C. A. S. in Crystallography and Biophysics, University of Madras, Guindy, Chennai 600 025, India.
Abstract:
The crystal structure of the tetradecanucleotide sequence d(CCCCGGTACCGGGG)(2) has been determined at 2.5 Å resolution in the tetragonal space group P4(1). This sequence was designed with the expectation of a four-way junction. However, the sequence crystallized as an A-DNA duplex and represents more than one full turn of the A-helix. The crystallographic asymmetric unit consists of one tetradecanucleotide duplex. The structural parameters of the A-type DNA duplex structure and the crystal-packing arrangement are described. One Mn(2+) ion was identified with direct coordination to the N7 position of G(13) and a water molecule at the major-groove side of the C(2)·G(13) base pair.
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