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Updated: May 23, 2026

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
Published on: December 31, 2017
Factors associated with mutans streptococci among young WIC-enrolled children
Karin Weber-Gasparoni1, Bryce M Goebel, David R Drake
1Pediatric Dentistry, University of Iowa, Iowa City, IA 52242-1001, USA. karin-weber@uiowa.edu. Karin
Insights
Mutans streptococci (MS) presence in young children is linked to increased dental caries and plaque. Factors like nonwhite race and lower maternal education are associated with higher MS rates.
Area of Science:
- Pediatric Dentistry
- Microbiology
- Public Health
Background:
- Mutans streptococci (MS) are key pathogens in early childhood caries.
- Understanding risk factors for MS colonization is crucial for prevention strategies.
Purpose of the Study:
- To investigate the relationship between mutans streptococci (MS) and various factors in children aged 12-49 months.
- To identify predictors of MS presence in a vulnerable pediatric population.
Main Methods:
- Cross-sectional analysis of 411 children from the WIC program.
- MS detection using RODAC plates; dental examinations for caries and plaque.
- Maternal questionnaires on demographics, diet, hygiene, and behaviors.
Main Results:
- MS was detected in 35% of children.
- Positive associations found between MS and nonwhite race, number of teeth, cavitated lesions, plaque presence, and lower maternal education.
- Beverage consumption, dental caries, and plaque measures showed bivariate relationships with MS.
Conclusions:
- MS colonization in young children is associated with higher caries and plaque levels.
- Lower maternal education and nonwhite racial background are significant risk factors for MS presence.
- Findings highlight the need for targeted oral health interventions for at-risk groups.
Objectives:
The objective of this study is to assess relationships between the presence or absence of mutans streptococci (MS) and other covariates in children aged 12-49 months.
Methods:
Data were analyzed using baseline information from 411 children enrolled in the Special Supplemental Food Program for Women, Infants and Children (WIC) who participated in a psychoeducational study in Iowa. Children were assessed for MS using a semiquantitative method (RODAC plates). Dental examinations using d(1) d(2) .(3) criteria and presence of visible plaque on incisors and molars were completed. Mothers completed a series of detailed questionnaires regarding their child's oral health, their socioeconomic status, their child's dietary/oral hygiene habits, and beverage consumption, among other behaviors. Bivariate relationships with the presence of MS were assessed and followed by multivariable modeling using logistic regression (alpha = 0.05).
Results:
MS was present in 144 children (35 percent). Bivariate relationships with MS presence were identified for multiple covariates that included demographic characteristics, type of beverage consumption, dental caries, and plaque measures. Multivariate logistic regression modeling suggested that MS presence in children was positively associated with nonwhite race of the child, number of teeth present, presence of cavitated lesions, proportion of teeth with plaque, and lower levels of maternal education.
Conclusions:
The presence of MS was associated with greater caries and plaque scores and with low maternal education and nonwhite racial background.
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