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Updated: May 23, 2026
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Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
Published on: February 7, 2022
Thawed solvent/detergent-treated plasma: too precious to be wasted after 6 hours?
Mareike Kristina Keller1, Axel Pruss, Michael Sander
1Department of Anaesthesiology and Intensive Care Medicine, Charité-Universitätsmedizin Berlin, Campus Virchow-Klinikum and Campus Charité Mitte, Berlin, Germany. mareike.keller@charite.de
Blood Transfusion = Trasfusione Del Sangue
|April 18, 2012
Summary
Thawed solvent/detergent-treated plasma (S/D-plasma) is stable for up to 24 hours at 4°C, ensuring availability of essential clotting factors for trauma patients. This stability is crucial for timely hemostatic agent administration in emergencies.
Area of Science:
- Transfusion Medicine
- Hematology
- Critical Care
Background:
- Trauma-induced coagulopathy necessitates prompt hemostatic agent administration.
- Solvent/detergent-treated plasma (S/D-plasma) availability is often delayed due to thawing requirements.
- The long-term stability of clotting factors in thawed S/D-plasma requires thorough investigation.
Purpose of the Study:
- To evaluate the stability of clotting factors and inhibitors in thawed S/D-plasma.
- To assess the impact of storage at 4°C for up to 6 days on S/D-plasma quality.
Main Methods:
- Analyzed 20 units of thawed S/D-plasma for clotting factor levels and bacterial contamination.
- Measured concentrations of fibrinogen, Factor II, V, VII, VIII, IX, X, XI, XIII, antithrombin, von Willebrand antigen (VWF-Ag), plasmin inhibitor, protein C, and free protein S over time.
- Utilized quantitative assays to determine factor concentrations and assess bacterial presence.
Main Results:
- Most clotting factors remained stable for up to 24 hours at 4°C.
- Significant decreases were observed in Fibrinogen, FII, FV, FVII, FVIII, FXIII, free protein S, and plasmin inhibitor after 6 days.
- Factors VII and VIII showed critical drops exceeding 20% or nearing quality thresholds after 24 hours; no bacterial contamination was detected.
Conclusions:
- Thawed S/D-plasma maintains stable clotting factors for up to 24 hours when stored at 4°C.
- Extended storage beyond 24 hours leads to a decline in critical hemostatic factors.
- Refrigerated storage of thawed S/D-plasma can enhance its availability for emergency use.
