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Cellular interactions of surface modified nanoporous silicon particles.

Luis M Bimbo1, Mirkka Sarparanta, Ermei Mäkilä

  • 1Division of Pharmaceutical Technology, Faculty of Pharmacy, University of Helsinki, FI-00014, Finland. luis.bimbo@helsinki.fi

Nanoscale
|April 18, 2012
PubMed
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Hydrophobin class II (HFBII) coating enhances porous silicon nanoparticles

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Drug Delivery Systems

Background:

  • Thermally hydrocarbonized porous silicon (THCPSi) nanoparticles are hydrophobic.
  • Hydrophobin class II (HFBII) is a protein known for its amphipathic properties.

Purpose of the Study:

  • To investigate the self-assembly of HFBII on THCPSi nanoparticles.
  • To evaluate the impact of HFBII coating on nanoparticle properties and drug delivery capabilities.

Main Methods:

  • HFBII coating of THCPSi nanoparticles.
  • Quantification of HFBII adsorption using BCA assay and HPLC.
  • Assessment of cell viability and cellular association in HT-29 and Caco-2 cells.
  • Evaluation of indomethacin permeation across Caco-2 monolayers.

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  • Stability assessment of radiolabeled HFBII on nanoparticles.

    • Main Results:

    • HFBII coating rendered hydrophobic THCPSi nanoparticles more hydrophilic.
    • Improved cell viability and enhanced cellular association of coated nanoparticles.
    • No significant effect of HFBII coating on indomethacin permeation.
    • Stable adsorption of HFBII on nanoparticles, suggesting potential for imaging applications.

    Conclusions:

    • HFBII coating improves wettability and biocompatibility of THCPSi nanoparticles.
    • Coated nanoparticles show potential for enhanced intestinal association in drug delivery.
    • HFBII-coated THCPSi nanoparticles are promising for drug delivery and imaging applications.