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Related Concept Videos

S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
The Cell Cycle Control System01:28

The Cell Cycle Control System

The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...

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Updated: May 23, 2026

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
12:02

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

Developing S-phase control.

Robert J Duronio1

  • 1Department of Biology, Department of Genetics, Lineberger Comprehensive Cancer Center, Program in Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill, NC 27599, USA. duronio@med.unc.edu

Genes & Development
|April 18, 2012
PubMed
Summary
This summary is machine-generated.

Cyclin-dependent kinase 1 (Cdk1) down-regulation triggers late-replicating DNA and longer S-phase duration in developing Drosophila embryos. This reveals Cdk1

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Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
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Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
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Visualizing Single-Stranded DNA Foci in the G1 Phase of the Cell Cycle
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Visualizing Single-Stranded DNA Foci in the G1 Phase of the Cell Cycle

Published on: December 22, 2023

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genomics

Background:

  • Early embryonic S phase duration is typically short.
  • S phase lengthens during development due to late-replicating genomic regions.
  • The regulation of S phase length during development is not fully understood.

Purpose of the Study:

  • To investigate the role of cyclin-dependent kinase 1 (Cdk1) in regulating S phase duration.
  • To identify the molecular triggers for the appearance of late-replicating DNA during development.
  • To understand Cdk1's function in controlling DNA replication timing in Drosophila embryos.

Main Methods:

  • Analysis of S phase duration in Drosophila embryos at different developmental stages.
  • Assessing the impact of Cdk1 activity modulation on DNA replication timing.
  • Identifying genomic regions that replicate late in development.

Main Results:

  • Down-regulation of Cdk1 activity correlates with the onset of late-replicating DNA.
  • Reduced Cdk1 activity leads to a significant increase in S-phase length.
  • Cdk1 plays a critical role in controlling the transition to longer S-phase durations.

Conclusions:

  • Cdk1 activity is a key regulator of DNA replication timing during embryonic development.
  • The down-regulation of Cdk1 is a critical event triggering the establishment of late-replicating DNA.
  • This study uncovers a novel role for Cdk1 in developmental replication control.