Related Experiment Video
Updated: May 23, 2026
07:38
Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames
Published on: April 11, 2019
The PRINTS database: a fine-grained protein sequence annotation and analysis resource--its status in 2012
Teresa K Attwood1, Alain Coletta, Gareth Muirhead
1Faculty of Life Sciences, The University of Manchester, Manchester M13 9PT, UK. teresa.k.attwood@manchester.ac.uk
Database : the Journal of Biological Databases and Curation
|April 18, 2012
Summary
The PRINTS database offers protein family fingerprints for identifying protein functions and relationships. Recent updates enhance usability and accessibility of these diagnostic sequence motifs.
Area of Science:
- Bioinformatics
- Computational Biology
- Structural Biology
Background:
- The PRINTS database catalogs protein family 'fingerprints', which are conserved motif groups.
- These fingerprints serve as distinctive signatures for protein families and domains.
Purpose of the Study:
- To report the current status of the PRINTS database.
- To introduce recent developments enhancing the usability and accessibility of annotation and analysis tools.
Main Methods:
- The database utilizes conserved motifs identified through multiple sequence alignments.
- Inter-relationships between motifs form unique signatures for classification.
Main Results:
- The February 2012 release (version 42.0) contains 2156 fingerprints and 12,444 motifs.
- The collection covers diverse protein types, including globular, membrane, and modular proteins.
Conclusions:
- PRINTS aids in assigning uncharacterized sequences to known families.
- It facilitates the inference of functional, structural, and evolutionary relationships.
Related Concept Videos
02:26Protein Networks
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
02:26Protein Networks
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
02:04Protein-protein Interfaces
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
02:24Ribosome Profiling
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
02:11Proteins: From Genes to Degradation
Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick. Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA molecules by RNA...
Transcription is the synthesis of RNA molecules by RNA...
02:47Protein Families
Protein families are groups of homologous proteins; that is, they have similarities in amino acid sequences and three-dimensional structures. Protein families usually occur because of gene duplication, where an additional copy of a gene is inserted into the genome of an organism. Mutations that change the amino acids but still allow the protein to be properly synthesized, will lead to new protein family members. If these new proteins contain similar amino acids in key locations, protein...