Related Experiment Video
Updated: May 23, 2026
08:58
Murine Kidney Transplant Technique
Published on: October 20, 2015
Donor-derived peripheral mononuclear cell DNA is associated with stable kidney allograft function: a randomized
Ghasem Solgi1, Joannis Mytilineos, Vijayakrishna Gadi
1Immunology Department, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Chimerism
|April 18, 2012
Summary
Donor bone marrow infusion (DBMI) in kidney transplant patients significantly increases microchimerism, a marker associated with stable graft function. Higher microchimerism levels correlate with infused cell dose, suggesting a role in transplant success.
Area of Science:
- Transplantation immunology
- Molecular biology
Background:
- Peripheral donor cell chimerism's link to kidney transplant tolerance is inconsistent.
- Previous studies lacked sensitivity in detecting microchimerism.
Purpose of the Study:
- To investigate the association between microchimerism and stable graft function in kidney transplant recipients.
- To compare microchimerism levels in patients receiving donor bone marrow infusion (DBMI) versus controls.
Main Methods:
- Prospective monitoring of 40 renal transplant patients (20 DBMI, 20 controls) for peripheral blood microchimerism.
- Utilized highly sensitive donor polymorphism-specific quantitative real-time PCR assays.
- Assessed microchimerism frequency and concentration.
Main Results:
- DBMI patients showed significantly higher frequency (95% vs. 58%) and concentration (115 vs. 13 genomes/million) of microchimerism.
- Stable graft function was more frequent in DBMI patients with microchimerism (94% vs. 50%).
- A significant correlation was found between infused cell dose and post-transplant microchimerism levels.
Conclusions:
- Highly sensitive assays reveal a strong association between microchimerism and stable graft function in kidney transplant recipients, particularly those undergoing DBMI.
- Microchimerism presence and quantity are linked to successful graft outcomes.
Related Concept Videos
01:28Kidney Transplant I: Introduction
A kidney transplant is a surgical approach that involves replacing a non-functioning kidney with a healthy one from a donor. This procedure is often a treatment option for end-stage renal disease (ESRD) patients. The method requires careful recipient selection, including evaluating various medical and psychosocial factors. These criteria vary between transplant centers but generally include assessments of the patient's overall health, adherence to medical recommendations, and lifestyle...
01:22Bone Marrow Sampling and Transplants
Bone marrow transplant is a potential cure for several diseases, including cancer and specific genetic disorders. Notably, this procedure is applicable for patients suffering from aplastic anemia, certain types of leukemia, severe combined immunodeficiency disease (SCID), Hodgkin's disease, non-Hodgkin's lymphoma, multiple myeloma, thalassemia, sickle-cell disease, and certain cancers.
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy the...
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy the...
01:26Kidney Transplant II: Surgical Procedure
Preoperative ManagementThe primary goals of preoperative management in kidney transplantation are to optimize the patient’s metabolic state and prepare them for surgery through diet adjustments, necessary dialysis, and tailored medical treatment. This phase also involves comprehensive infection screening and patient education about the surgical procedure and postoperative care to improve outcomes and adherence.Medical ManagementA comprehensive evaluation is required for both the living donor...