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Published on: June 17, 2015
[Screening and identification of natural antisense transcript in mouse cerebral cortex]
Ping Li1, Jing Zhang, Ai-Hua Li
1National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Summary
Natural antisense transcripts (NATs) were found in mouse neocortex development. These NATs may play a role in regulating coding genes during cerebral cortex development.
Area of Science:
- Genomics
- Neuroscience
Context:
- The mouse neocortex is crucial for higher cognitive functions.
- Understanding gene regulation in the neocortex is vital for developmental studies.
Purpose:
- To screen and identify natural antisense transcripts (NATs) in the mouse neocortex.
- To investigate the existence of NATs during cerebral cortex development.
Summary:
- Bioinformatic screening identified 31 potential NATs in mouse neocortex layer-specific genes.
- RT-PCR confirmed 8 of these as NAT positive, validating their existence.
- Sequencing further characterized the identified NATs.
Impact:
- This study confirms the presence of NATs in the developing mouse neocortex.
- NATs may influence mouse cortical development by regulating associated coding genes.
