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Updated: May 23, 2026

Transillumination-Assisted Dissection of Specific Stages of the Mouse Seminiferous Epithelial Cycle for Downstream Immunostaining Analyses
Published on: October 7, 2020
[Separation and morphological observation of mouse testis seminiferous tubule epithelium]
1National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Objective:
To explore a simple and feasible technique to locate proteins during spermatogenesis.
Methods:
Various germ cells and somatic cells were separated by collagenase I and DNase I after the albuginea was removed. The cells were then smeared, dyed, and observed directly under fluorescence and confocal microscopy.
Results:
Germ cells at different steps were successfully identified by specific dyestuffs for acrosome and nucleolus.
Conclusion:
A simple method for locating proteins during spermatogenesis was successfully developed.
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