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Related Experiment Video

Updated: May 23, 2026

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
11:31

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Published on: July 4, 2018

Mast cell degranulation in human periodontitis.

Shiguang Huang1, Fangli Lu, Ying Chen

  • 1Department of Periodontology, Faculty of Dentistry, Jinan University School of Medicine, Guangzhou, China. thshg@126.com

Journal of Periodontology
|April 19, 2012
PubMed
Summary

Mast cell degranulation is linked to increased severity in human periodontal disease. Higher mast cell densities correlate with more advanced periodontitis, suggesting a role in disease progression.

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Published on: July 2, 2013

Area of Science:

  • Immunology
  • Periodontology
  • Cell Biology

Background:

  • Mast cells are immune cells involved in allergic and inflammatory responses.
  • Their role in periodontal diseases, especially chronic non-allergic inflammation like periodontitis, is understudied.
  • This study investigates mast cell degranulation in relation to periodontitis progression.

Purpose of the Study:

  • To analyze the relationship between mast cell degranulation and the progression of human periodontal disease.
  • To quantify mast cell densities and degranulation in different stages of periodontitis.

Main Methods:

  • Histopathological analysis of 50 clinical specimens (healthy, moderate, and advanced periodontitis).
  • Staining with hematoxylin and eosin, toluidine blue (for mast cells), and immunohistochemistry (for tryptase).
  • Quantification of total and degranulated mast cell densities and tryptase-positive mast cells.

Main Results:

  • Significantly increased total and degranulated mast cell densities in moderate and advanced periodontitis compared to controls (P <0.01).
  • Elevated densities of tryptase-positive mast cells in periodontitis groups, increasing with disease severity.
  • Advanced periodontitis showed significantly higher mast cell densities and degranulation than moderate periodontitis (P <0.01).

Conclusions:

  • A significant correlation exists between tryptase-positive mast cell density, degranulation, and periodontitis severity.
  • Mast cell degranulation is associated with the progression of human periodontal disease.