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Engineering Streptomyces tenebrarius to synthesize single component of carbamoyl tobramycin
1College of Biological Science and Technology, Fuzhou University, Fujian, China. hongwr56@163.com
Aims:
To engineer Streptomyces tenebrarius for producing carbamoyl tobramycin as a main component.
Methods And Results:
The aprH-M gene fragment (apramycin biosynthetic gene from GenBank) in S. tenebrarius Tt49 was knocked out by genetic engineering to form S. tenebrarius T106 (ΔaprH-M). Compared to the wild-type strain, mutant strain T106 (ΔaprH-M) no longer produced apramycin, while mainly synthesize carbamoyl tobramycin. TLC and HPLC-MS analyses indicated that the mutant strain significantly increased the production of carbamoyl tobramycin.
Conclusions:
The metabolic flow for the apramycin and its analogues biosynthesis was blocked by disrupting the aprH-M gene clusters. The aprH-M gene clusters might be essential for the biosynthesis of apramycin. The mutant strain T106 mainly synthesized carbamoyl tobramycin.
Significance And Impact Of Study:
The mutant T106 mainly produces carbamoyl tobramycin without synthesizing apramycin, which will reduce cost of postextraction from fermentation products. Therefore, it has good prospects for industrial application.
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