Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mechanism of Lamellipodia Formation

Mechanism of Lamellipodia Formation

Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...

Mechanism of Filopodia Formation

Mechanism of Filopodia Formation

Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...

Cancer Cell Migration through Invadopodia

Cancer Cell Migration through Invadopodia

Invadosome is a broad category of cell surface structures with proteolytic activity that degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However, invadopodia can...

Regulation of Nuclear Protein Sorting

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...

Coat Assembly and GTPases

Coat Assembly and GTPases

Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...

The JAK-STAT Signaling Pathway

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

LASP1, a Novel Protein in Spermatozoa and Acrosome Reaction.

Molecular reproduction and development·2026

Same author

Steroid pulse therapy for acute T-cell-mediated rejection after kidney transplantation: mechanisms, evidence, and unresolved questions.

Frontiers in immunology·2026

Same author

A Novel Pre-Kidney Transplant Risk Score to Optimize Waiting List Management.

Journal of clinical medicine·2026

Same author

Induction of fibrosis in human kidney organoids delineates mechanisms and therapeutic targets of fibrotic kidney disease.

Stem cell research & therapy·2026

Same author

Early detection of glycocalyx and microvascular damage in suspected sepsis in the emergency department: the EDGE study.

Critical care (London, England)·2026

Same author

The APOL1 variant p.N264K is predicted to block ion flow by occluding a pore at the cell surface.

Life science alliance·2026

Search research articles

Related Experiment Video

Updated: May 23, 2026

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Published on: January 11, 2017

Lasp-1 regulates podosome function.

Miriam Stölting¹, Christiane Wiesner, Vanessa van Vliet

¹Medizinische Klinik D, Abteilung für Molekulare Nephrologie, Universitätsklinikum Münster, Münster, Germany.

|April 20, 2012

Summary

This summary is machine-generated.

Lasp-1 is identified as a new component of podosomes, dynamic cell structures involved in matrix remodeling. This actin-binding protein regulates podosome assembly and function, impacting cell behavior.

More Related Videos

In vivo and in vitro Studies of Adaptor-clathrin Interaction

In vivo and in vitro Studies of Adaptor-clathrin Interaction

Published on: January 26, 2011

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

Related Experiment Videos

Last Updated: May 23, 2026

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Published on: January 11, 2017

In vivo and in vitro Studies of Adaptor-clathrin Interaction

In vivo and in vitro Studies of Adaptor-clathrin Interaction

Published on: January 26, 2011

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

Area of Science:

Cell Biology
Cytoskeleton Dynamics
Molecular Cell Biology

Background:

Eukaryotic cells utilize adhesive structures for environmental interaction and motility regulation.
Podosomes are dynamic actin-rich structures involved in matrix remodeling and degradation, distinct from classical focal adhesions.
Lasp-1, an actin-binding protein, regulates cytoskeleton architecture and cell migration, with known presence at focal adhesions and lamellipodia.

Purpose of the Study:

To investigate the role of Lasp-1 in the formation and function of podosomes.
To determine if Lasp-1 is a component of podosome structures and influences their dynamics.

Main Methods:

Immunofluorescence microscopy to assess Lasp-1 localization within podosomes.
Live cell imaging to observe Lasp-1 recruitment during podosome assembly.
siRNA-mediated knockdown of Lasp-1 in human macrophages to evaluate effects on podosome dynamics and matrix degradation.

Main Results:

Lasp-1 localizes to the podosome ring structure, colocalizing with known podosome proteins like zyxin and vinculin.
Lasp-1 is recruited during the early stages of podosome assembly.
Lasp-1 knockdown impairs podosome dynamics and reduces their matrix degradation capacity in macrophages.

Conclusions:

Lasp-1 is a novel component of podosomes.
Lasp-1 plays a regulatory role in podosome assembly and function, including matrix degradation.