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Anionic Polymerization of an Amphiphilic Copolymer for Preparation of Block Copolymer Micelles Stabilized by π-π Stacking Interactions
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Structural properties induced by the composition of biocompatible phospholipid-based microemulsion and amphotericin B

Cristina Maria Franzini1, Kelly Christina Pestana, Eduardo Ferreira Molina

  • 1Universidade Estadual Paulista, UNESP, Faculdade de Ciências Farmacêuticas, Programa de Pós-graduação em Ciências Farmacêuticas, Rodovia Araraquara-Jaú km 01, 14801-902, Araraquara, SP, Brazil.

Journal of Biomedical Nanotechnology
|April 21, 2012
PubMed

Insights

Anionic microemulsions (MEs) effectively carry amphotericin B, with droplet size influenced by surfactant and oil content. Formulation composition dictates drug loading and system structure for optimized drug delivery.

Area of Science:

  • Colloid and Surface Chemistry
  • Pharmaceutical Nanotechnology
  • Drug Delivery Systems

Background:

  • Anionic microemulsions (MEs) are explored as potential nanocarriers for drug delivery.
  • The interaction between drug molecules and ME components influences carrier performance.
  • Amphotericin B is an antifungal agent often requiring effective delivery systems.

Purpose of the Study:

  • To investigate anionic microemulsions (MEs) formulated with soya phosphatidylcholine, Tween-20, and sodium oleate as drug carriers for amphotericin B.
  • To characterize the structural and physicochemical properties of these MEs.
  • To understand the influence of ME composition on amphotericin B incorporation and system behavior.

Main Methods:

  • Microemulsion formulation using soya phosphatidylcholine, Tween-20, sodium oleate, and cholesterol.
  • Characterization techniques including photon correlation spectroscopy, rheological behavior analysis, and polarized light microscopy.
  • Investigation of amphotericin B incorporation and its dependence on microemulsion composition.

Main Results:

  • Droplet diameters increased with amphotericin B incorporation.
  • Surfactant concentration stabilized droplets, while increased oil phase led to larger droplet diameters.
  • Amphotericin B incorporation increased with oil phase and surfactant concentration, reaching a plateau.
  • Rheological analysis indicated pseudoplastic behavior with slight thixotropy.
  • Increased oil-to-surfactant ratio induced lamellar arrangements in microemulsions.

Conclusions:

  • Anionic microemulsions are viable carriers for amphotericin B, with tunable properties based on formulation.
  • The pseudo-phase model effectively describes amphotericin B distribution within the microemulsion system.
  • Formulation composition significantly impacts microemulsion structure and drug loading capacity.

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