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Updated: May 23, 2026

Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes
Published on: December 19, 2020
Recent developments in bacterial protein glycan coupling technology and glycoconjugate vaccine design
Vanessa S Terra1, Dominic C Mills1, Laura E Yates1
1London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
Abstract:
The discovery of the Campylobacter jejuni N-linked glycosylation system combined with its functional expression in Escherichia coli marked the dawn of a new era in glycoengineering. The process, termed protein glycan coupling technology (PGCT), has, in particular, been applied to the development of glycoconjugate vaccines. In this review, we highlight recent technical developments in this area, including the first structural determination of the coupling enzyme PglB, the use of glycotags for optimal glycan attachment and the possible applications of other glycosylation systems and how these may improve and extend PGCT.
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