Related Experiment Video
Updated: May 23, 2026

11:40
A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
Published on: November 14, 2018
Ionic liquids promote PCR amplification of DNA
Yugang Shi1, Yen-Liang Liu, Peng-Yeh Lai
1Department of Chemistry and Biochemistry, National Chung Cheng University, Minhsiung, Chiayi 62102, Taiwan, ROC.
Abstract:
A bicyclic imidazolium ionic liquid (4d), [b-4C-im][Br], was found to be highly effective not only for promoting PCR of GC-rich DNA by minimizing non-specific amplification, but also for facilitating PCR of normal-GC DNA under mild conditions.
More Related Videos
Related Concept Videos
DNA Isolation
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
PCR
Overview
PCR - Polymerase Chain Reaction
Overview

