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A sensitive and reliable detection of thrombin via enzyme-precipitate-coating-linked aptamer assay

Hye-Jin Lee1, Byoung Chan Kim, Min-Kyu Oh

  • 1Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, Republic of Korea.

Chemical Communications (Cambridge, England)
|April 21, 2012
PubMed

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.