Selective labeling of apomorphine receptors by 3H-LSD

|June 15, 1979

Summary

This study differentiates two dopamine receptor types based on drug affinity. Findings suggest distinct receptors for 3H-apomorphine and 3H-LSD binding, supporting separate dopamine receptor classifications.

Area of Science:

Neuropharmacology
Receptor Binding Assays

Background:

Dopamine receptors exhibit varying affinities for dopamine.
Two main receptor types are identified: high-affinity (3H-dopamine/3H-apomorphine) and low-affinity (3H-neuroleptic).

Purpose of the Study:

To specifically label and characterize the 3H-apomorphine/dopamine receptor site.
To investigate the relationship between 3H-apomorphine and 3H-LSD binding sites.

Main Methods:

Utilized calf caudate tissue for receptor binding studies.
Employed specific blocking agents (phentolamine, serotonin, spiperone) to isolate receptor interactions.
Compared the inhibitory effects of dopaminergic drugs on 3H-LSD and 3H-apomorphine binding.

Main Results:

Dopaminergic drugs inhibited binding of both 3H-LSD and 3H-apomorphine at similar concentrations.
This inhibition occurred in the presence of agents blocking alpha-adrenergic, serotonergic, and neuroleptic receptors.

Conclusions:

The results support the existence of separate dopamine receptor sites.
The 3H-apomorphine/dopamine receptor and the 3H-neuroleptic/dopamine receptor are distinct entities.

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Selective labeling of apomorphine receptors by 3H-LSD

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Related Experiment Videos

Selective labeling of apomorphine receptors by 3H-LSD

Area of Science:

Background:

Purpose of the Study:

Main Methods:

Main Results:

Conclusions:

Related Concept Videos

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