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Cytoskeletal interactions at the nuclear envelope mediated by nesprins
Surayya Taranum1, Ilknur Sur, Rolf Müller
1Institute of Biochemistry I, Medical Faculty, University of Cologne, Joseph-Stelzmann-Strasse 52, 50931 Cologne, Germany.
International Journal of Cell Biology
|April 21, 2012
Summary
Nesprin proteins form a nuclear envelope network connecting the nucleus to cytoskeletal filaments. This study reveals Nesprin-1 interacts with Nesprin-3, linking the nucleus to both actin and intermediate filament networks.
Area of Science:
- Cell Biology
- Molecular Biology
- Cytoskeletal Dynamics
Background:
- Nesprin-1 is a giant nuclear envelope protein linking the nucleus to the actin cytoskeleton via its N-terminal F-actin binding domain and C-terminal spectrin repeats.
- Nesprin-1 interacts with nuclear envelope proteins like emerin and lamin.
- Nesprin family members connect the nucleus to different cytoskeletal networks.
Purpose of the Study:
- To investigate the interaction between Nesprin-1 and Nesprin-3.
- To elucidate the role of Nesprin-3 in cytoskeletal network connections to the nucleus.
- To propose a model for Nesprin-mediated nuclear-cytoskeletal connections.
Main Methods:
- Focus on N-terminal Nesprin-1 sequences for interaction studies.
- Ectopic expression of Nesprin-3 in COS7 cells.
- In vitro assays to demonstrate F-actin interaction of Nesprin-3.
Main Results:
- Nesprin-1 N-terminal sequences interact with Nesprin-3.
- Ectopic Nesprin-3 expression recruits vimentin filaments to the nucleus in COS7 cells.
- Evidence for Nesprin-3 interaction with F-actin in vitro.
Conclusions:
- Nesprins interact with each other to form a nuclear-associated network.
- Nesprin-3 connects the nucleus to the intermediate filament network.
- The Nesprin network integrates the nucleus with multiple cytoskeletal systems (actin and intermediate filaments).
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