[The preparation and properties of catechol-1,2-dioxygenase from Pseudomonas putida]

Catechol-1,2-dioxygenase (EC 1.13.11.1) catalyzes the degradation of catechol to cis, cis-muconic acid. The biochemical properties of catechol-1,2-dioxygenase from Pseudomonas putida 84103 were investigated. The optimum pH and temperature is 7.5-8.0 and 25-30 degrees C, respectively. Cu2+, Zn2+ inhibit the enzyme activity. The paper chromatograph and UV absorption spectrum of enzymatic reaction product are accordance with those of the standard muconic acid.