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Proteomic profiling of thyroid papillary carcinoma
Yoshiyuki Ban1, Gou Yamamoto, Michiya Takada
1Division of Diabetes, Metabolism and Endocrinology, Department of Medicine, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8666, Japan.
Journal of Thyroid Research
|April 21, 2012
Summary
Researchers identified new protein markers, nephronectin (NPNT) and malectin (MLEC), specific to papillary thyroid carcinoma (PTC). These findings could aid in diagnosing this common endocrine malignancy.
Area of Science:
- Endocrinology
- Oncology
- Proteomics
Background:
- Papillary thyroid carcinoma (PTC) is the most prevalent endocrine malignancy.
- Accurate diagnostic markers are crucial for effective management.
Purpose of the Study:
- To identify novel protein biomarkers for papillary thyroid carcinoma (PTC).
- To compare protein expression profiles between PTC and normal thyroid tissue.
Main Methods:
- Shotgun liquid chromatography/tandem mass spectrometry (LC/MS/MS) was employed on protein extracts.
- Real-time PCR (RT-PCR) and immunohistochemistry (IHC) were used for validation.
Main Results:
- A total of 524 proteins were detected in PTC and 432 in normal thyroid tissue.
- 145 proteins were specific to PTC, and 53 were specific to normal thyroid tissue.
- Nephronectin (NPNT) and malectin (MLEC) were identified as potential new markers, with overexpression confirmed in PTC via IHC.
Conclusions:
- NPNT and MLEC show promise as characteristic protein markers for papillary thyroid carcinoma.
- These identified markers warrant further investigation for clinical application in PTC diagnosis.
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