MicroRNAs and lymph node metastatic disease in lung cancer

Francesco Fazi1, Giulia Fontemaggi

  • 1Department of Medico-Surgical Sciences and Biotechnologies, Sapienza University of Rome, Corso della Repubblica 79, 04100 Latina, Italy. francesco.fazi@uniroma1.it

Related Concept Videos

Modeling Metastatic Endometrial Cancer Model: A Technique to Generate Endometrial Cancer with Lymph Node Metastasis in Rabbit Models03:23

Modeling Metastatic Endometrial Cancer Model: A Technique to Generate Endometrial Cancer with Lymph Node Metastasis in Rabbit Models

This video demonstrates the generation of a rabbit model of endometrial cancer by injecting a highly metastatic VX2 cell suspension into the sutured uterine horns of the rabbit. This leads to generation of primary endometrial cancer with retroperitoneal lymph node...
2.6K
Lymph Node Exam08:06

Lymph Node Exam

Source: Richard Glickman-Simon, MD, Assistant Professor, Department of Public Health and Community Medicine, Tufts University School of Medicine, MA
The lymphatic system has two main functions: to return extracellular fluid back to the venous circulation and to expose antigenic substances to the immune system. As the collected fluid passes through lymphatic channels on its way back to the systemic circulation, it encounters multiple nodes consisting of highly concentrated clusters of...
416.9K
Sentinel Lymph Node Mapping and Biopsy for Endometrial Cancer at Early Stage with Laparoscopy05:52

Sentinel Lymph Node Mapping and Biopsy for Endometrial Cancer at Early Stage with Laparoscopy

This protocol describes the identification and resection of sentinel lymph nodes to make the operation as easy and minimally invasive as...
13.0K
Quantification of Tumor Cell Adhesion in Lymph Node Cryosections06:09

Quantification of Tumor Cell Adhesion in Lymph Node Cryosections

Here, we describe a simple and inexpensive method that allows the quantification of adhesive tumor cells to lymph node (LN) cryosections. LN-adherent tumor cells are readily identified by light microscopy and confirmed by a fluorescence-based method, giving an adhesion index that reveals the tumor cell-binding affinity to LN...
13.3K
Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species12:53

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

This protocol provides an overview of procedures for the isolation of RNA for the transcriptomic profiling of lymph node tissues from large animals, including steps in the identification and excision of lymph nodes from livestock and wildlife, sampling approaches to provide consistency across multiple animals, and considerations plus representative results for the post-collection preservation and processing for RNA...
28.5K
Non-surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry08:01

Non-surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry

We illustrate non-surgical delivery of test materials into the lungs of anesthetized mice via the trachea. This method permits lung exposure to bacterial and viral pathogens, cytokines, antibodies, beads, chemicals, or dyes. We further describe harvesting and processing of lungs and lung draining lymph nodes (LDLNs) for flow cytometry.
60.5K