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Related Experiment Video

Updated: May 23, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

Guanacastane-type diterpenoids from Coprinus radians.

Yi-xin Ou¹, Yao-yao Li, Xiao-ming Qian

¹Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, Xiamen, Fujian 361005, PR China.

|April 24, 2012

Summary

Thirteen new guanacastane-type diterpenoids were isolated from the fungus Coprinus radians. Radianspene C demonstrated significant antitumor activity against the MDA-MB-435 cell line with an IC50 of 0.91 μM.

Related Experiment Videos

Last Updated: May 23, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

Area of Science:

Natural Products Chemistry
Mycology
Pharmacology

Background:

The higher fungal strain Coprinus radians M65 produces a variety of secondary metabolites.
Guanacastane skeleton-based compounds are of interest for their potential biological activities.

Purpose of the Study:

To isolate and characterize new diterpenoids from Coprinus radians M65.
To evaluate the antitumor activity of the isolated compounds.

Main Methods:

Isolation and structure elucidation of thirteen diterpenoids (radianspenes A-M) using chromatographic and spectroscopic techniques.
Antitumor activity evaluation using the MDA-MB-435 cell line.

Main Results:

Thirteen diterpenoids, including three lactams and one dimer, were identified.
Radianspene C exhibited potent inhibitory activity against MDA-MB-435 cells with an IC50 value of 0.91 μM.

Conclusions:

Coprinus radians M65 is a source of novel guanacastane-type diterpenoids.
Radianspene C shows promising antitumor potential and warrants further investigation.