Related Experiment Video
Updated: May 23, 2026

Direct Analysis of Single Cells by Mass Spectrometry at Atmospheric Pressure
Published on: September 4, 2010
A novel approach to signal normalisation in atmospheric pressure ionisation mass spectrometry
Michael Vogeser1, Fabian Kirchhoff, Roland Geyer
1Institute of Clinical Chemistry, University of Munich, Munich, Germany. michael.vogeser@med.uni-muenchen.de
Abstract:
The aim of our study was to test an alternative principle of signal normalisation in LC-MS/MS. During analyses, post column infusion of the target analyte is done via a T-piece, generating an "area under the analyte peak" (AUP). The ratio of peak area to AUP is assessed as assay response. Acceptable analytical performance of this principle was found for an exemplary analyte. Post-column infusion may allow normalisation of ion suppression not requiring any additional standard compound. This approach can be useful in situations where no appropriate compound is available for classical internal standardisation.
Related Concept Videos
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview
Chemical Ionization (CI) Mass Spectrometry
Tandem Mass Spectrometry
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences
Mass Spectrometry: Overview
Electrospray Ionization (ESI) Mass Spectrometry
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
