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Inhibitory effect of 5,8,11-eicosatrienoic acid on angiogenesis.

T Hamazaki¹, T Nagasawa, K Hamazaki

¹Department of Clinical Sciences, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama City, Toyama, Japan. hamazaki@inm.u-toyama.ac.jp

Prostaglandins, Leukotrienes, and Essential Fatty Acids

|April 24, 2012

Summary

n-9 eicosatrienoic acid (20:3n-9) inhibits blood vessel formation (angiogenesis). This fatty acid, found in cartilage, may offer new treatments for conditions with excessive vasculature.

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Area of Science:

Biochemistry
Cell Biology
Vascular Biology

Background:

Cartilage has high levels of n-9 eicosatrienoic acid (20:3n-9).
Cartilage lacks blood vessels, suggesting 20:3n-9 may inhibit angiogenesis.

Purpose of the Study:

To investigate the effect of 20:3n-9 on angiogenesis.
To determine if 20:3n-9 can inhibit vascular endothelial growth factor-A (VEGF-A) stimulated angiogenesis.

Main Methods:

Co-culture of human umbilical vein endothelial cells and diploid fibroblasts.
Stimulation with VEGF-A and various fatty acids (0.1-10 μmol/L).
Measurement of vessel area after 10 days via immunostaining and image analysis.

Main Results:

20:3n-9 and n-3 eicosatrienoic acid (20:3n-3) dose-dependently inhibited VEGF-A-stimulated angiogenesis.
Other tested fatty acids (arachidonic, eicosapentaenoic, 20:3n-6, oleic) did not inhibit angiogenesis.
Arachidonic and 20:3n-6 acids enhanced angiogenesis without VEGF-A.

Conclusions:

The high concentration of 20:3n-9 in avascular cartilage may be linked to its vessel-free nature.
20:3n-9 shows potential therapeutic value for treating disorders characterized by excessive blood vessel growth.