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Related Experiment Video

Updated: May 23, 2026

Use of the MicroSiM (µSiM) Barrier Tissue Platform for Modeling the Blood-Brain Barrier

Use of the MicroSiM (µSiM) Barrier Tissue Platform for Modeling the Blood-Brain Barrier

Published on: January 12, 2024

Macroporous silicon chips for laterally resolved, multi-parametric analysis of epithelial barrier function.

Stefanie Michaelis¹, Christina E Rommel, Jan Endell

¹Institut fuer Analytische Chemie, Chemo- & Biosensorik, Universitaet Regensburg, Regensburg, Germany.

|April 24, 2012

Summary

This novel assay visualizes cell layer permeability at subcellular resolution. It reveals defects and permeation routes, offering new insights into epithelial and endothelial barrier function.

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Last Updated: May 23, 2026

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Published on: January 12, 2024

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Reconstituting Cytoarchitecture and Function of Human Epithelial Tissues on an Open-Top Organ-Chip

Reconstituting Cytoarchitecture and Function of Human Epithelial Tissues on an Open-Top Organ-Chip

Published on: February 17, 2023

Area of Science:

Biotechnology
Cell Biology
Materials Science

Background:

Epithelial and endothelial cell layers form critical biological barriers.
Assessing the permeability of these barriers at high resolution is essential for understanding physiological and pathological processes.

Purpose of the Study:

To develop and validate a novel assay for visualizing macromolecular permeability of cell layers.
To achieve subcellular lateral resolution in permeability analysis.
To simultaneously assess cell layer tightness using impedance analysis.

Main Methods:

Utilizing silicon chips with densely packed, ordered, dead-ended pores (μm-diameters).
Growing cells on the porous chip surface, using pores as femtolitre cuvettes for probe accumulation.
Employing gold coating for impedance analysis of inorganic ion permeability.

Main Results:

The assay visualizes macromolecular permeability with μm lateral resolution.
Defects in cell layers and permeation routes of solutes are clearly revealed.
Simultaneous impedance measurements provide data on cell layer tightness.

Conclusions:

The developed assay offers an unprecedented view of epithelial and endothelial barrier function.
This method allows detailed characterization of cell layer permeability and integrity.
The combined permeability and impedance analysis provides comprehensive barrier assessment.