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Related Concept Videos

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The Nucleolus

The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
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Related Experiment Video

Updated: May 23, 2026

HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells
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Specific small nucleolar RNA expression profiles in acute leukemia.

W Valleron1, E Laprevotte, E-F Gautier

  • 1INSERM UMR1037-Cancer Research Center of Toulouse, University of Toulouse III, Toulouse, France.

Leukemia
|April 24, 2012
PubMed
Summary

Small nucleolar RNAs (snoRNAs) show altered expression in leukemia, with specific signatures in acute promyelocytic leukemia (APL). SNORD114-1 snoRNA influences cell cycle regulation, suggesting a role for snoRNAs in cancer development.

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Published on: September 1, 2019

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Non-coding RNA regulation in cancer, beyond microRNAs, remains largely unexplored.
  • Small nucleolar RNAs (snoRNAs) are a class of non-coding RNAs whose role in cancer is not well understood.

Purpose of the Study:

  • To investigate snoRNA expression patterns in acute leukemias.
  • To identify specific snoRNA signatures associated with different types of acute leukemia.
  • To explore the functional role of specific snoRNAs in leukemogenesis and cell cycle regulation.

Main Methods:

  • Microarray analysis to assess global snoRNA expression.
  • High-throughput quantitative PCR (qPCR) for specific snoRNA validation.
  • In vitro studies on acute promyelocytic leukemia (APL) cells.
  • Analysis of cell cycle modulation and relevant pathways (Rb/p16).

Main Results:

  • SnoRNA expression patterns are significantly altered in leukemic cells compared to normal controls.
  • A distinct snoRNA signature, involving SNORD112-114, was identified in APL.
  • Transcription of these snoRNAs is linked to the PML-RARalpha fusion protein and differentiation status.
  • SNORD114-1 promotes cell growth by modulating the cell cycle via the Rb/p16 pathway.

Conclusions:

  • SnoRNA regulation differs between normal and cancer cells.
  • Chromosomal translocations can impact snoRNA gene expression.
  • SnoRNAs play a role in cell cycle regulation and potentially in cancer development.