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Serotonin-storing secretory vesicles.

H Tamir1, M D Gershon

  • 1Department of Anatomy and Cell Biology, Columbia University College of Physicians & Surgeons, New York, New York 10032.

Annals of the New York Academy of Sciences
|January 1, 1990
PubMed
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Thyroid parafollicular cells, used as a model for neuronal 5-HT storage, possess vesicles with chloride channels. These channels regulate vesicle acidification, impacting serotonin transport and potentially influencing neuronal function.

Area of Science:

  • Cell Biology
  • Neuroscience
  • Endocrinology

Background:

  • Thyroid parafollicular cells serve as a model for studying 5-HT (serotonin) storage in neurectodermal cells.
  • These cells share neuronal properties and can undergo neuronal differentiation induced by nerve growth factor (NGF).

Purpose of the Study:

  • To characterize the 5-HT storage organelles in thyroid parafollicular cells.
  • To investigate the role of chloride channels and proton pumps in regulating the internal pH of these vesicles.
  • To examine the function of the 5-HT binding protein (SBP) in these storage vesicles.

Main Methods:

  • Isolation and characterization of parafollicular 5-HT storage vesicles.
  • In vitro studies involving secretogogue stimulation and manipulation of ion gradients.

Related Experiment Videos

  • Measurement of vesicle acidification and 3H-5-HT transport.
  • Utilizing medullary thyroid carcinoma (MTC) cells for studying SBP.

    • Main Results:

    • Parafollicular vesicle membranes contain a chloride channel gated by secretogogues, facilitating vesicle acidification via H+ ATPase.
    • Vesicle acidification is limited by membrane potential (delta psi) when chloride conductance is low.
    • Transmembrane transport of 3H-5-HT is inhibited by dissipation of the proton gradient (delta pH).
    • Parafollicular vesicles, similar to neuronal synaptic vesicles, contain a specific 5-HT binding protein (SBP).

    Conclusions:

    • Thyroid parafollicular vesicles exhibit a regulated mechanism for internal pH modification, influencing 5-HT storage and transport.
    • The presence of SBP in these vesicles and in MTC cells suggests its conserved role in serotonin storage.
    • Further research is needed to determine the physiological significance of these findings in both normal parafollicular cells and MTC.