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Related Experiment Video

Updated: May 23, 2026

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

Dental pulp stem cells from primary teeth quality analysis: laboratory procedures.

R Saha¹, S Tandon, R Rajendran

¹Pedodontics & Preventive Dentistry, ITS-CDSR, Muradnagar. rooposhi@yahoo.com

The Journal of Clinical Pediatric Dentistry

|April 25, 2012

Summary

Stem cells isolated from inflamed primary teeth pulp can differentiate into multiple lineages, showing similar surface markers to bone marrow stem cells. Oral tissue isolation requires precise techniques.

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Last Updated: May 23, 2026

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

Primary Culture of Dental Pulp Stem Cells

Primary Culture of Dental Pulp Stem Cells

Published on: May 5, 2023

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth

Published on: May 17, 2024

Area of Science:

Regenerative Medicine
Stem Cell Biology
Oral Biology

Background:

Dental pulp stem cells (DPSCs) are a promising source for regenerative therapies.
Inflamed primary teeth offer a potential source of DPSCs.

Purpose of the Study:

To detail the isolation, processing, and differentiation of stem cells from inflamed primary dental pulp.
To compare DPSCs with bone marrow stem cells.

Main Methods:

Dental pulp tissue was collected from primary teeth undergoing pulp therapy.
Cells were cultured, isolated, and induced for in vitro differentiation.
Cell surface markers of DPSCs were analyzed and compared to bone marrow.

Main Results:

No significant differences in surface marker expression were observed between DPSCs and bone marrow stem cells.
Stem cells derived from dental pulp successfully differentiated into multiple lineages.

Conclusions:

Stem cells can be successfully isolated and differentiated from inflamed primary dental pulp.
The isolation process for oral stem cells is technique-sensitive.