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Updated: May 23, 2026

Digital Microfluidics for Automated Proteomic Processing
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Digital microfluidics.

Kihwan Choi1, Alphonsus H C Ng, Ryan Fobel

  • 1Department of Chemistry, University of Toronto, Toronto, Ontario M5S 3H6, Canada.

Annual Review of Analytical Chemistry (Palo Alto, Calif.)
|April 25, 2012
PubMed
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Digital microfluidics (DMF) offers precise control over tiny liquid droplets for chemical analysis. This review covers DMF droplet actuation, fabrication, and applications in analytical chemistry.

Area of Science:

  • Analytical Chemistry
  • Microfluidics
  • Lab-on-a-chip technology

Background:

  • Digital microfluidics (DMF) is an innovative liquid-handling technology.
  • It allows precise manipulation of picoliter- to microliter-sized droplets on electrode arrays.
  • Droplets serve as independent micro-reactors for various chemical processes.

Purpose of the Study:

  • To provide a comprehensive review of digital microfluidics (DMF) technology.
  • To highlight its significance and applications within analytical chemistry.
  • To discuss the underlying principles, fabrication, and integration of DMF devices.

Main Methods:

  • Review of existing literature on digital microfluidics.
  • Detailed explanation of droplet actuation theory (e.g., electrowetting-on-dielectric).

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  • Discussion of device fabrication techniques and integration strategies.

    • Main Results:

    • DMF enables controlled movement, merging, splitting, and dispensing of droplets.
    • Advantages include simple instrumentation, flexible device design, and compatibility with other technologies.
    • Diverse applications in analytical chemistry are demonstrated.

    Conclusions:

    • Digital microfluidics is a powerful platform for automated chemical analysis.
    • Its unique features facilitate miniaturization and high-throughput screening.
    • Continued advancements promise broader adoption across scientific disciplines.