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Updated: May 23, 2026

A Computer-assisted Multi-electrode Patch-clamp System

A Computer-assisted Multi-electrode Patch-clamp System

Published on: October 18, 2013

Automated multi-slice extracellular and patch-clamp experiments using the WinLTP data acquisition system with

William W Anderson¹, Stephen M Fitzjohn, Graham L Collingridge

¹MRC Centre for Synaptic Plasticity, School of Physiology and Pharmacology, University of Bristol, University Walk, Bristol BS8 1TD, UK. w.w.anderson@bristol.ac.uk

Journal of Neuroscience Methods

|April 25, 2012

Summary

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WinLTP software now automates perfusion control for synaptic plasticity experiments. This enhancement allows for fully automated multi-slice and patch-clamp recordings, improving experimental efficiency and accuracy.

Area of Science:

Neuroscience
Computational Neuroscience
Physiology

Background:

Previous versions of WinLTP automated electrical stimulation and data acquisition for synaptic plasticity studies.
Manual perfusion solution changes limited the automation of multi-slice and patch-clamp experiments.
Accurate and rapid solution exchange is critical for certain electrophysiology experiments.

Purpose of the Study:

To introduce automated perfusion control capabilities into the WinLTP software.
To enable full automation of multi-slice extracellular and single-cell patch-clamp experiments.
To enhance the efficiency and accuracy of synaptic plasticity research.

Main Methods:

Integration of a 'Perfuse' event into the WinLTP Protocol Builder for scripting perfusion changes.

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Last Updated: May 23, 2026

A Computer-assisted Multi-electrode Patch-clamp System

A Computer-assisted Multi-electrode Patch-clamp System

Published on: October 18, 2013

Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices

Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices

Published on: July 31, 2017

Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters

Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters

Published on: February 3, 2018

Utilizing the 'Perfuse' event and digital/analog output control for rapid solution changes during patch-clamp recordings.

Implementation of stepper control for dual- or triple-line perfusion systems, supporting up to 48 solutions.

Main Results:

Automated perfusion control is now available for both slice and patch-clamp experiments within WinLTP.
The 'Perfuse' event allows for perfusion changes between and during sweeps, enhancing experimental flexibility.
Stepper-controlled perfusion enables complex multi-solution exchange for advanced patch-clamp applications.

Conclusions:

The addition of automated perfusion control significantly advances the automation capabilities of WinLTP.
This integration streamlines synaptic plasticity experiments, reducing manual intervention and improving data quality.
WinLTP now offers a comprehensive solution for automated electrophysiological recordings.