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Related Experiment Video

Updated: May 23, 2026

Developmental Toxicity Assay Based on Real-Time Monitoring of Fibroblast Growth Factor Signal Disruption in Human Induced Pluripotent Stem Cells
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Developmental Toxicity Assay Based on Real-Time Monitoring of Fibroblast Growth Factor Signal Disruption in Human Induced Pluripotent Stem Cells

Published on: October 10, 2025

Evaluating biotoxicity with fibroblasts derived from human embryonic stem cells.

Xiaoying Wang1, Shenglin Li, Tong Cao

  • 1Central Laboratory, Peking University School and Hospital of Stomatology, China.

Toxicology in Vitro : an International Journal Published in Association with BIBRA
|April 25, 2012
PubMed
Summary

Differentiated human embryonic stem cells (EBf-H9) show promise as a new cell model for toxicity testing. These cells effectively detected cytotoxicity and genotoxicity from Sodium fluoride and Formaldehyde exposure.

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Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing

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Area of Science:

  • Stem cell biology
  • Toxicology
  • Biomaterials science

Background:

  • Human embryonic stem cells (hESCs) offer a renewable source for cell-based research.
  • Developing reliable cellular models for toxicity screening is crucial for biomaterial and chemical safety assessments.

Purpose of the Study:

  • To evaluate differentiated fibroblasts from hESCs (EBf-H9 cells) as a cellular model for cytotoxicity and genotoxicity screening.
  • To assess the utility of EBf-H9 cells in response to Sodium fluoride (NaF) and Formaldehyde (FA) exposure.

Main Methods:

  • EBf-H9 cells were derived from hESCs (H9) via embryonic body (EB) formation.
  • Cells were analyzed for proliferation (MTT assay), gene/protein expression (RT-PCR, immunocytochemistry), and karyotype.
  • Cytotoxicity was assessed using MTT assay and flow cytometry.
  • Genotoxicity was evaluated via micronucleus test (MNT), sister chromatid exchange (SCE), and comet assay.

Main Results:

  • EBf-H9 cells exhibited fibroblast morphology, a diploid karyotype, and expressed fibroblast markers (prolyl 4-hydroxylase β, vimentin) while downregulating pluripotency markers (Oct-4, Sox-2, Nanog).
  • NaF and FA inhibited EBf-H9 cell proliferation and induced cell cycle arrest, similar to murine L929 cells.
  • Genotoxicity assays demonstrated positive responses in EBf-H9 cells upon NaF and FA treatment, comparable to the L929 cell line.

Conclusions:

  • EBf-H9 cells represent a viable and comparable alternative to murine L929 cells for toxicity screening.
  • This hESC-derived fibroblast model shows significant potential for future research in biomaterial and chemical agent toxicity assessments.