[Preparation of human minisatellite DNA probes]

A 23-mer oligonucleotide based on the core sequence was chemically synthesized and used to screen the human genomic library. Fifteen positive recombinants containing the minisatellite sequences were identified, and one of them, C35.9, was used to perform Southern hybridization with the DNAs from unrelated Chinese individuals. Each sample has 3-11 hybridizing bands, and some of which are polymorphic. The band patterns detected under controlled condition are individual-specific in a limited population. This indicates that the minisatellites obtained by screening the library can be used to detect the polymorphisms of the minisatellites.