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[Studies on polymorphism of C6 in Han nationality of China]
Insights
This study investigated C6 complement component polymorphism in Han Chinese populations. Gene frequencies of C6*A, C6*B, and C6*R variants were determined, revealing variations across different regions.
Area of Science:
- Genetics
- Immunology
- Population Studies
Background:
- The complement system, particularly C6, plays a crucial role in immune responses.
- Understanding genetic polymorphism in complement proteins is vital for population genetics and disease association studies.
Purpose of the Study:
- To analyze the genetic polymorphism of the C6 complement component in three distinct Han Chinese populations.
- To compare C6 gene frequencies with those reported in other ethnic groups worldwide.
Main Methods:
- Polyacrylamide gel isoelectric focusing (PAGIF) was employed to separate C6 protein variants.
- Immunoblotting techniques were utilized for precise identification and quantification of C6 alleles.
Main Results:
- Gene frequencies for C6*A, C6*B, and C6*R (including C6*B2) were determined for Zhangzhou, Chengdu, and Harbin populations.
- Observed C6*A frequencies in Han Chinese were generally below 0.5, consistent with Mongoloid populations.
- C6*B2 allele frequencies in the Han Chinese samples ranged from 0.0073 to 0.0396, aligning with Mongoloid populations and differing from Caucasian frequencies.
Conclusions:
- The study provides valuable data on C6 gene frequencies within Han Chinese populations.
- The findings contribute to the understanding of global C6 polymorphism distribution across different ethnic groups.
- Regional variations in C6 allele frequencies highlight the importance of population-specific genetic analyses.
Abstract:
The polymorphism of C6 in three groups of Han nationality was investigated by using polyacrylamide gel isoelectric focusing and immunoblotting technique. The gene frequencies obtained are as follows: Zhangzhou City (Fujiang Province) C6*A:0.4634, C6*B:0.5000, C6*R:0.0366 (C6*B2:0.0317); Chendu City (Sichuan Province) C6*A:0.4975, C6*B:0.4480, C6*R:0.0545 (C6*B2:0.0396); Harbin City (Heilongjiang Province) C6*A:0.4708, C6*B:0.5219, C6*R:0.0073 (C6*B2:0.0073). The frequency of C6*A in Mongoloid populations is usually lower than 0.5, while that of black people ranges between 0.5 and 0.6 and that of white people is higher than 0.6. The other difference between Caucasian and Mongoloid is that the former has very few C6*B2 while the frequency of C6*B2 in the latter ranges from 0.03 to 0.07.