Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Rapid and precise whole blood protamine titration.

Y Y Fu¹, C L Teng, V C Yang

¹College of Pharmacy, University of Michigan, Ann Arbor 48109-1065.

ASAIO Transactions

|July 1, 1990

Summary

A new whole blood protamine titration method uses azure A dye for rapid, accurate heparin reversal assessment. This colorimetric assay simplifies clinical use during surgery, improving patient safety.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[Application of D-type stoma repair in parastomal hernia after permanent sigmoidostomy].

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery·2024

Same author

The clinical applications of D-type parastomal hernia repair surgery.

Hernia : the journal of hernias and abdominal wall surgery·2024

Same author

[Feasibility study of R method of gastrojejunostomy applied to Billroth II digestive tract reconstruction after laparoscopic radical distal gastrectomy].

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery·2023

Same author

[Using the external auditory canal skin graft of the healthy side to treat unilateral congenital aural atresia: preliminary results of clinical research].

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2022

Same author

[The upadate classification system of congenital auricular malformation].

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2021

Same author

[Correction of severe cup ears using postauricular tongue-shaped flap combined with deep fascial suspension].

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2021

Area of Science:

Biochemistry
Clinical Chemistry
Hematology

Background:

Protamine titration is crucial for reversing heparin anticoagulation during surgery.
Existing clotting assays for protamine titration are time-consuming and complex.
A need exists for a faster, more accessible method for intraoperative protamine assessment.

Purpose of the Study:

To develop and validate a rapid, precise whole blood protamine titration method.
To replace traditional clotting assays with a simpler colorimetric approach.
To enable real-time protamine dose estimation for effective heparin reversal.

Main Methods:

Development of a novel protamine titration assay utilizing azure A dye as the indicator.
Application of a colorimetric method for determining the titration end-point.
Validation of the method using whole blood specimens.

Main Results:

The developed method provides accuracy comparable to existing clotting assays.
The assay significantly reduces processing time compared to traditional methods.
The colorimetric approach offers simplicity, flexibility, and speed.

Conclusions:

The rapid whole blood protamine titration method is accurate and efficient.
This facile colorimetric assay can be readily implemented in clinical settings.
The method facilitates precise protamine dosing for heparin reversal in operating rooms.

Related Experiment Videos