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Impact of 4HPR on the expression of E-Cad in human bladder transitional epithelial cancer cells T24
Eyou Wang1,2, Jun Li1, Guohua Yang1
1School of Basic Medical Science, Wuhan University, Wuhan, 430071, China.
Abstract:
Previous researches showed that the expression level of E-Cad in most infiltrating cancer cells was reduced or negative. This study explored whether 4HPR restrained the infiltration of bladder cancer cells through regulating the expression of E-Cad. The infiltrating bladder cancer cells T24 were cultured, and then treated by a proper dosage of drug. Their viability was a determined by MTT method. Western blotting and RT-PCR were adopted to detect the changes of E-Cad gene expression at both protein and mRNA levels. Moreover, immunofluorescent staining and confocal fluorescence microscopy were employed for the observation of the expression of E-Cad. The result showed that, at both mRNA and protein levels, the expression level of E-Cad in T24 cells treated by 4HPR was significantly higher than that of control group, while the β-Cat expression was also relocated from the cell nucleus to cytoplasm. Our findings suggested that the regulatory function of 4HPR on infiltration of bladder cancer cells T24 is at least partly achieved by regulating the expression of E-Cad.
Insights
4-HPR treatment significantly increased E-Cadherin (E-Cad) expression in bladder cancer cells. This suggests 4-HPR may inhibit cancer cell infiltration by regulating E-Cad expression.
Area of Science:
- Oncology
- Cell Biology
Background:
- Reduced E-Cadherin (E-Cad) expression is observed in most infiltrating cancers.
- Understanding E-Cad regulation is crucial for targeting cancer cell infiltration.
Purpose of the Study:
- To investigate if 4-HPR (4-hydroxyphenyl retinoic acid) restrains bladder cancer cell infiltration.
- To determine if 4-HPR regulates E-Cadherin expression in T24 bladder cancer cells.
Main Methods:
- Cell viability assessed using MTT assay.
- E-Cadherin gene expression analyzed via Western blotting and RT-PCR.
- Immunofluorescent staining and confocal microscopy used to observe E-Cadherin expression.
Main Results:
- 4-HPR treatment significantly upregulated E-Cadherin at both mRNA and protein levels in T24 cells.
- Beta-catenin (β-Cat) expression was observed to relocate from the nucleus to the cytoplasm following 4-HPR treatment.
Conclusions:
- 4-HPR demonstrates a regulatory role in bladder cancer cell infiltration.
- The mechanism involves, at least partly, the modulation of E-Cadherin expression by 4-HPR.
